Vol. 3 No. 2 (2020): International Journal of Aging Research
Research Articles

Study of Variables Involved in Male Infertility Identified in the Spermograms Assessed in Assisted Human Reproduction

Bárbara Stefany da Silva Souza1*, Evandro Valentim da Silva2, Sérgio Antônio Santos da Costa e Silva1, Ana Maria Medeiros de Ataídes3, Fálba Bernadete Ramos dos Anjos1, Adriana Fracasso4
1UNIVERSIDADE FEDERAL DE PERNAMBUCO; 2HOSPITAL DAS CLÍNICAS/UFPE; 3UNIVERSIDADE DE PERNAMBUCO; 4CLÍNICA NASCER/PE.
Keywords
  • age groups, azoospermia, semen.
How to Cite
Bárbara Stefany da Silva Souza1*, Evandro Valentim da Silva2, Sérgio Antônio Santos da Costa e Silva1, Ana Maria Medeiros de Ataídes3, Fálba Bernadete Ramos dos Anjos1, Adriana Fracasso4. (2020). Study of Variables Involved in Male Infertility Identified in the Spermograms Assessed in Assisted Human Reproduction. International Journal of Aging Research, 3(2), 62. https://doi.org/10.28933/ijoar-2020-03-1105

Abstract

Introduction: According to the World Health Organization, about 8 to 10% of couples worldwide have infertility problems and male internal aspects are the main reasons for half of occurrences of human sterility. Through the spermogram, it is possible to qualitatively and quantitatively analyze semen, contributing to the diagnosis of male fertile state.

Objective: To study the relationship among sperm viscosity, concentration, motility and volume parameters and male infertility factors and to show the influence of the subject age on these seminal parameters.

Methodology: A survey was conducted in the male infertility database of the Nascer Clinic (Recife / Pernambuco) of men aged 27 to 61 years, with a history of marital infertility, from 2018 to 2019. The subjects studied were grouped into categories according to the classification of the seminal parameters analyzed (volume, concentration, motility and viscosity) in their sperm. Student’s t-test was used for normal distribution and Mann-Whitney test for non-normal using the GraphPad Prism 8 program.

Results: Among the studied individuals, there was a significant difference (p <0.05) between the populations with obstructive azoospermia and nonobstructive azoospermia and among the percentages of oligozoospermic individuals with obstructive azoospermia. The azoospermia group had a significantly higher average age than the normozoospermia group.

Conclusions: This suggests that azoospermia is present in older men, compromising male fertility. Sperm testing should be performed by all men of childbearing age to investigate possible changes in the genesis of gametic cells.

References

  1. Graner V, Barros S. Maternal complications and neonatal events associated to multiple pregnancies resulting from assisted reproduction techniques. Rev. Esc. Enferm. USP [Inter-net]. Mar.2009; 43(1):103-9. Available from: http://www.periodicos.usp.br/reeusp/article/view/40332.
  2. Pasqualotto EB, Pasqualotto FF. Espermograma e testes de função espermática. Rev. Feminina. [Internet]. Fev.2006; 34(2):91-98. Available from: https://pesquisa.bvsalud.org/portal/resource/pt/lil-434313?lang=pt
  3. Roupa Z., Polikandrioti M., Sotiropoulou P., Faros E., Koulouri A., Wozniak G. et al. Causes of infertility in women at reproductive age. Health Science Journal. Medicine-Gynecology and obstetrics: 2009. 80-87.
  4. Johnson L, Grumbles JS, Bagheri A, Petty CS. Increased germ cell degeneration during postprophase of meiosis is related to increased serum follicle-stimulating hormone concen-trations and reduced daily sperm production in aged men. Rev. Biology of reproduction [In-ternet]. December.1989; 42(2): 281-287. Available from: https://academic.oup.com/biolreprod/article/42/2/281/2764302
  5. Kühnert B, Nieschlag E. Reproductive functions of the ageing male. Rev. Human reproduc-tion update [Internet]. July.2004; 10(4): 327-339. Available from: https://academic.oup.com/humupd/article/10/4/327/635699
  6. Nieschlag E, Lammers U, Freischem CW, Langer K, Wickings EJ. Reproductive functions in young fathers and grandfathers. The Journal of Clinical Endocrinology & Metabolism. 1982. 676-681.
  7. Santos JTC. Fertilidade masculina e envelhecimento. Coimbra: Faculdade de Medicina da Universidade de Coimbra, 2015. Dissertação de mestrado.
  8. Santos MS. A utilização da injeção intracitoplasmática de espermatozoide no ovócito como recurso na infertilidade masculina. Brasília: Faculdade de Ciências da Educação e Saúde do Centro Universitário de Brasília, 2016. Trabalho de Conclusão de Curso.
  9. Neri QV, Lee B, Rosenwaks Z, Machaca K, Palermo GD. Understanding fertilization through intracytoplasmic sperm injection (ICSI). Rev. Cell calcium [Internet]. January.2014; 55 (1): 24-37. Available from: https://www.sciencedirect.com/science/article/pii/S0143416013001449
  10. Duarte, HDS. Fatores preditivos de sucesso na colheita cirúrgica de espermatozoides na azoospermia não obstrutiva. Porto: Instituto de Ciências Biomédicas Abel Salazar da Uni-versidade do Porto, 2018. Dissertação de mestrado.
  11. Mourthé FA, Faria ARL, Melo UB, Taitson RE. Extração de espermatozóide testicular (TESE) em Síndrome de “somente células de Sertoli”. Jornal Brasileiro de Reprodução Assistida. 2001.24-26.
  12. Pasqualotto FF. Investigação e reprodução assistida no tratamento da infertilidade mas-culina. Rev. Brasileira de Ginecologia e obstetrícia [Internet]. Fevereiro.2007; 29(2): 103-112. Available from: http://www.scielo.br/pdf/%0D/rbgo/v29n2/08.pdf
  13. Arent A, Telöken C, Hartmann A, Badalotti M, Petracco R et al. Resultados de la inyección intracitoplasmática de espermatozoides en hombres con azoospermia no obstructiva: uti-lidad de la biopsia testicular previa. Rev. Colomb Obstet Ginecol [Internet]. December.2006; 57(4): 245-255. Available from: http://www.scielo.org.co/scielo.php?pid=S003474342006000400003&script=sci_arttext&tlng=pt
  14. Hu M, Zhang Y, Ma H, Ernest HY, Wu XK. Eastern medicine approaches to male infertility. Rev. Semin Reprod Med [Internet]. 2013; 31(4): 301-310. Available from: https://www.thiemeconnect.com/products/ejournals/html/10.1055/s-0033-1345589
  15. Turek PJ. Practical approaches to the diagnosis and management of male infertility. Rev. Nat Clin Pract Urol [Internet]. 2005; 2(5):226-38. Available from: https://www.nature.com/articles/ncpuro0166
  16. Shefi S, Turek PJ. Definition and current evaluation of subfertile men. Rev. Int Braz J Urol [In-ternet]. 2006; 32(4):385-97. Available from: http://www.scielo.br/scielo.php?pid=s1677-55382006000400002&script=sci_arttext
  17. Carrara RC, Yamasaki R, Braganca W, Raskin S, Sartorato EL, Pina JMN. Etiologic investi-gations on male infertility before intracytoplasmatic sperm injection (ICSI). Rev. Genet Couns [Internet]. 2006; 17(3):385-389. Available from: https://repositorio.usp.br/item/001592448.
  18. Centola GM, Eberly S. Seasonal variations and age-related changes in human sperm count, motility, motion parameters, morphology, and White blood cell concentration. Rev. Fertility and Sterility [Internet]. 1999; 72(5):803-808. Available from: https://www.sciencedirect.com/science/article/pii/S0015028299003957
  19. Kidd AS, Eskenazi B, Wyrobek J. Effects of male age on semen quality and fertility: a re-view of the literature. Rev. Fertility and Sterility [Internet]. 2001; 75(2):237-248. Available from: https://www.sciencedirect.com/science/article/pii/S0015028200016794
  20. Chen Z, Toth T, Godfrey LB, Mercedat N, Schiff I, Hauser R. Seasonal Variation and Age-Related Changes in Human Semen Parameters. Journal of Andrology. 2003.226-231.
  21. Eskenazi B, Wyrobek AJ, Sloter E, Kidd AS, Moore L, Young S. et al. The association of age and semen quality in healthy men. Rev. Human Reproduction [Internet]. 2003; 18(2): 447-454. Available from: https://academic.oup.com/humrep/article/18/2/447/639258
  22. Carlsen E, Giwercman A, Keiding N, Skakkebaek NE. Evidence for decreasing quality of semen during past 50 years. British Medical Journal. 1992. 609-613.
  23. Krause W, Habermann B. No change with age in semen volume, sperm count and sperm mo-tility in individual men consulting an infertility clinic. Rev. Urologia internationalis [Internet]. 2000; 64(3):139-142. Available from: https://www.karger.com/Article/Abstract/30514
  24. Pinto, MALA. Impacto da idade na fertilidade masculina. Porto: Instituto de Ciências Biomé-dicas Abel Salazar da Universidade do Porto, 2017. Dissertação de Mestrado.
  25. Cavalcante MB, Rocha MP, Dias MLCM, Dias OJQ, Souza DOA, Roberto, IG. Interferência da idade sobre a qualidade seminal. Rev. bras. ginecol. Obstet [Internet]. 2008; 30(11):561-565. Available from: http://bases.bireme.br/cgibin/wxislind.exe/iah/online/?IsisScript=iah/iah.xis&src=google&base=ADOLEC&lang=p&nextAction=lnk&exprSearch=507277&indexSearch=ID
  26. AUGER J, Kunstmann JM, Czyglik R, Jouannet P. Decline in semen quality among fertile men in Paris during the past 20 years. New England Journal of Medicine. 1995. 281-285.
  27. Haidl G, Jung A, Schill WB. Ageing and sperm function. Rev. Human reproduction [Internet]. 1996; 11(3): 558-560. Available from: https://academic.oup.com/humrep/article/11/3/558/582495
  28. Andolz P, Bielsa MA, Vila J. Evolution of semen quality in North-eastern Spain: a study in 22 759 infertile men over a 36 year period. Rev. Human Reproduction [Internet]. 1999; 14(3):731-735, 1999. Available from: https://academic.oup.com/humrep/article/14/3/731/632912
  29. Irvine S, Cawood E, Richardson D, MacDonald E, Aitken J. Evidence of deteriorating semen quality in the United Kingdom: birth cohort study in 577 men in Scotland over 11 years. Rev. Bmj [Internet]. 1996; 312(7029): 467-471. Available from: https://www.bmj.com/content/312/7029/467.short
  30. Zinaman MJ, Brown CC, Selevan SG, Clegg ED. Semen quality and human fertility: a pro-spective study with healthy couples. Journal of Andrology, 2000. 145-153.